Construction and characterization of a chimeric virus (BIV/HIV-1) carrying the bovine immunodeficiency virus gag-pol gene
G Chen, S Wang, K Xiong, J Wang, T Ye, W Dong… - AIDS, 2002 - journals.lww.com
HIV-1 HXB2 5′ LTR region, most of BIV R29 gag-polsegment and HIV-1 HXB2 polIN—3′
LTR region were respectively amplified. A chimeric clone, designated as pHBIV 3753, was
constructed by cloning three fragments sequentially into pUC18. MT4 cells were transfected
with pHBIV 3753. The replication and expressions of the chimeric virus (HBIV 3753) were
monitored by RT activity and IFA. The results firstly demonstrated that it is possible to
generate a new type of the BIV/HIV-1 chimeric virus containing BIVgag-polgene.The …
LTR region were respectively amplified. A chimeric clone, designated as pHBIV 3753, was
constructed by cloning three fragments sequentially into pUC18. MT4 cells were transfected
with pHBIV 3753. The replication and expressions of the chimeric virus (HBIV 3753) were
monitored by RT activity and IFA. The results firstly demonstrated that it is possible to
generate a new type of the BIV/HIV-1 chimeric virus containing BIVgag-polgene.The …
Construction and Characterization of a Chimeric Virus (BIV/HIV-1) Carrying the Bovine Immunodeficiency Virus gag-pol Gene: Research Letters
G Chen, S Wang, K Xiong, J Wang, T Ye, W Dong… - 2002 - digitalcommons.unl.edu
Abstract HIV-1HXB2 5′ LTR region, most of BIVR29 gag-pol segment and HIV-1HXB2 pol
IN-3′ LTR region were respectively amplified. A chimeric clone, designated as pHBIV
3753, was constructed by cloning three fragments sequentially into pUC18. MT4 cells were
transfected with pHBIV 3753. The replication and expressions of the chimeric virus (HBIV
3753) were monitored by RT activity and IFA. The results firstly demonstrated that it is
possible to generate a new type of the BIV/HIV-1 chimeric virus containing BIV gag-pol …
IN-3′ LTR region were respectively amplified. A chimeric clone, designated as pHBIV
3753, was constructed by cloning three fragments sequentially into pUC18. MT4 cells were
transfected with pHBIV 3753. The replication and expressions of the chimeric virus (HBIV
3753) were monitored by RT activity and IFA. The results firstly demonstrated that it is
possible to generate a new type of the BIV/HIV-1 chimeric virus containing BIV gag-pol …
以上显示的是最相近的搜索结果。 查看全部搜索结果